The article examines a charity in London at the end of the 15th–16th Centuries based on the material of the wills of merchants and artisans and their widows. The directions of the citizens‘s charity are identified: Church, social, cultural and educational, and specific forms of their manifestation are characterized from a gender perspective. The author shows that deep social changes and Reformation processes in Tudor England also caused serious transformations of spiritual, religious and moral values of people of that time, which was reflected in the charitable activities of Londoners.